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Large Construct Sequencing

 Do you need an experienced Sanger sequencing service for larger constructs such as BACs, PACs, FOSMIDs, COSMIDs and genomic DNA? Then we suggest taking a closer look at our Large Construct Sequencing Service. Basically, we are able to offer you the following four services:

To learn more about this type of sequencing service, please see the various sections below. To obtain information about pricing, please enter our webshop and download the current price list or even better, contact our experts.

 

 

Partial Sequencing of BACs, PACs, FOSMIDs and COSMIDs

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With respect to partial sequencing of BACs, PACs, FOSMIDs and COSMIDs, you can choose between single sequencing runs and primer walking reactions. Moreover, you can either send us your isolated DNA of interest or your E. coli clones.

 

Main Service Features and Benefits:

  • Read length per run: up to 1100 bases

  • Standard primers and specific primers will be added free of charge.

  • Rapid turnaround time: sequencing Time <72h

  • Failed reactions will be repeated free of charge.

  • Additional service: BAC, PAC, FOSMID and COSMID DNA isolation from E. coli (extra charge will be necessary)

 

Sample Requirements for Isolated DNA Samples

Single Sequencing Run:

  • 2 µg DNA per reaction

Primer Walking:

  • 4 µg DNA/1000 bases (in case of single-stranded sequencing)

  • 8 µg DNA/1000 bases (in case of double-stranded sequencing)

Please note: We would appreciate receiving E. coli single clones of your construct as a back-up.

 

 

Partial Sequencing of Genomic DNA

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With respect to partial sequencing of genomic DNA, you can choose between single sequencing runs and primer walking reactions. To obtain good sequencing results, it is important that you provide us with genomic DNA of high quality which is not larger than 4-5 MB.

 

Main Service Features and Benefits:

  • Read length per run: up to 1100 bases

  • Standard primers and specific primers will be added free of charge

  • Rapid turnaround time: sequencing time <72h

  • Failed reactions will be repeated free of Charge

 

Sample Requirements for Isolated Genomic DNA Samples

Single Sequencing Run:

  • 2 µg DNA per reaction

Primer Walking:

  • 4 µg DNA/1000 bases (in case of single-stranded sequencing)

  • 8 µg DNA/1000 bases (in case of double-stranded sequencing)

 

 

Entire Sequencing of BACs, PACs, FOSMIDs and COSMIDs

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Entire sequencing on large constructs can be sequenced to x-fold defined coverage or publication quality either by conventional Sanger sequencing or by next generation sequencing approaches. Traditionally, this has been accomplished by first creating a shotgun library with fragments of defined sizes and then sequencing the obtained fragments. Nowadays, each project is evaluated individually.

Seqlab - Sequence Laboratories Göttingen GmbH
Hannah-Vogt-Str. 1
37085 Göttingen

Postfach 3343
37023 Göttingen

Telefon 0551-370 00 10
Telefax 0551-370 00 12

E-Mail info@seqlab.de

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